3BZB
Crystal structure of uncharacterized protein CMQ451C from the primitive red alga Cyanidioschyzon merolae
Experimental procedure
Experimental method | SINGLE WAVELENGTH |
Source type | SYNCHROTRON |
Source details | APS BEAMLINE 23-ID-D |
Synchrotron site | APS |
Beamline | 23-ID-D |
Temperature [K] | 100 |
Detector technology | CCD |
Collection date | 2007-11-08 |
Detector | MARMOSAIC 300 mm CCD |
Wavelength(s) | 0.97927 |
Spacegroup name | P 31 2 1 |
Unit cell lengths | 103.783, 103.783, 105.385 |
Unit cell angles | 90.00, 90.00, 120.00 |
Refinement procedure
Resolution | 46.554 - 2.790 |
R-factor | 0.212 |
Rwork | 0.209 |
R-free | 0.26100 |
Structure solution method | SAD |
RMSD bond length | 0.009 |
RMSD bond angle | 1.191 |
Data reduction software | DENZO |
Data scaling software | SCALEPACK |
Phasing software | SHARP |
Refinement software | REFMAC (5.2.0019) |
Data quality characteristics
Overall | Inner shell | Outer shell | |
Low resolution limit [Å] | 46.554 | 46.554 | 2.900 |
High resolution limit [Å] | 2.790 | 6.030 | 2.790 |
Rmerge | 0.126 | 0.053 | 0.705 |
Number of reflections | 16619 | ||
<I/σ(I)> | 11.232 | 1.487 | |
Completeness [%] | 99.4 | 99.9 | 94.8 |
Redundancy | 19.1 | 20.1 | 10.1 |
Crystallization Conditions
crystal ID | method | pH | temperature | details |
1 | VAPOR DIFFUSION, HANGING DROP | 277 | Protein solution (10 mg/mL Se-Met protein, 0.050 M Sodium chloride, 0.0003 M TCEP, 0.005 M MES pH 6.0) mixed in a 1:1 ratio with the Well solution (9% PEG 5000, 1.44 M Tetramethylammonium chloride, 0.10 M Triethanolamine pH 8.0 ). Cryoprotected with fomblin, VAPOR DIFFUSION, HANGING DROP, temperature 277K |