3BZ6
Crystal structure of a conserved protein of unknown function from Pseudomonas syringae pv. tomato str. DC3000
Replaces: 2NR3Experimental procedure
Experimental method | MAD |
Source type | SYNCHROTRON |
Source details | APS BEAMLINE 19-ID |
Synchrotron site | APS |
Beamline | 19-ID |
Temperature [K] | 100 |
Detector technology | CCD |
Collection date | 2006-08-13 |
Detector | ADSC QUANTUM 315 |
Wavelength(s) | 0.97929, 0.97943 |
Spacegroup name | P 65 |
Unit cell lengths | 72.025, 72.025, 73.119 |
Unit cell angles | 90.00, 90.00, 120.00 |
Refinement procedure
Resolution | 31.540 - 2.210 |
R-factor | 0.19788 |
Rwork | 0.196 |
R-free | 0.24085 |
Structure solution method | MAD |
RMSD bond length | 0.015 |
RMSD bond angle | 1.630 |
Data reduction software | HKL-3000 |
Data scaling software | HKL-3000 |
Phasing software | SHELXD |
Refinement software | REFMAC (5.2.0019) |
Data quality characteristics
Overall | Outer shell | |
Low resolution limit [Å] | 31.540 | 2.250 |
High resolution limit [Å] | 2.200 | 2.200 |
Rmerge | 0.091 | 0.391 |
Number of reflections | 10229 | |
<I/σ(I)> | 46 | 2.59 |
Completeness [%] | 93.1 | 58.5 |
Redundancy | 13.2 | 9.5 |
Crystallization Conditions
crystal ID | method | pH | temperature | details |
1 | VAPOR DIFFUSION, SITTING DROP | 277 | 0.1M Sodium acetate, 20% Isopropanol, 20% PEG 4000, VAPOR DIFFUSION, SITTING DROP, temperature 277K |