3BYQ
Crystal structure of a duf1185 family protein (bb2672) from bordetella bronchiseptica rb50 at 1.70 A resolution
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | SSRL BEAMLINE BL11-1 |
| Synchrotron site | SSRL |
| Beamline | BL11-1 |
| Temperature [K] | 100 |
| Detector technology | CCD |
| Collection date | 2007-12-05 |
| Detector | MARMOSAIC 325 mm CCD |
| Wavelength(s) | 0.97912 |
| Spacegroup name | C 2 2 21 |
| Unit cell lengths | 96.542, 133.127, 92.544 |
| Unit cell angles | 90.00, 90.00, 90.00 |
Refinement procedure
| Resolution | 29.854 - 1.700 |
| R-factor | 0.139 |
| Rwork | 0.138 |
| R-free | 0.16900 |
| Structure solution method | SAD |
| RMSD bond length | 0.015 |
| RMSD bond angle | 1.640 |
| Data reduction software | MOSFLM |
| Data scaling software | SCALA |
| Phasing software | SHELX |
| Refinement software | REFMAC (5.4.0067) |
Data quality characteristics
| Overall | Inner shell | Outer shell | |
| Low resolution limit [Å] | 29.854 | 29.854 | 1.740 |
| High resolution limit [Å] | 1.700 | 7.600 | 1.700 |
| Rmerge | 0.107 | 0.050 | 0.716 |
| Total number of observations | 3477 | 23099 | |
| Number of reflections | 65104 | ||
| <I/σ(I)> | 5.9 | 12.4 | 1.1 |
| Completeness [%] | 99.2 | 98.4 | 96.6 |
| Redundancy | 5 | 4.3 | 5 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, SITTING DROP | 9.5 | 277 | NANODROP, 20% PEG 8000, 0.1M CHES pH 9.5, VAPOR DIFFUSION, SITTING DROP, temperature 277K |
