3BYN
Crystal structure of B. subtilis levansucrase mutant E342A bound to raffinose
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | ROTATING ANODE |
| Source details | ENRAF-NONIUS FR591 |
| Temperature [K] | 100 |
| Detector technology | IMAGE PLATE |
| Collection date | 2003-03-05 |
| Detector | MAC Science DIP-2030 |
| Wavelength(s) | 1.5418 |
| Spacegroup name | P 21 21 21 |
| Unit cell lengths | 51.211, 66.700, 124.042 |
| Unit cell angles | 90.00, 90.00, 90.00 |
Refinement procedure
| Resolution | 30.000 - 2.100 |
| R-factor | 0.18144 |
| Rwork | 0.179 |
| R-free | 0.22357 |
| Structure solution method | MOLECULAR REPLACEMENT |
| Starting model (for MR) | 1oyg |
| RMSD bond length | 0.007 |
| RMSD bond angle | 1.066 |
| Data reduction software | DENZO |
| Data scaling software | SCALEPACK |
| Refinement software | REFMAC (5.1.19) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 62.020 | 2.100 |
| High resolution limit [Å] | 2.030 | 2.030 |
| Rmerge | 0.053 | 0.097 |
| Number of reflections | 27688 | |
| <I/σ(I)> | 25.1 | 25.5 |
| Completeness [%] | 98.2 | 98.1 |
| Redundancy | 5 | 4.5 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | MICRODIALYSIS | 6.3 | 291 | diammonium phosphate, sodium acetate, pH 6.3, MICRODIALYSIS, temperature 291K |
