3BYI
Crystal structure of human Rho GTPase activating protein 15 (ARHGAP15)
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | SLS BEAMLINE X10SA |
| Synchrotron site | SLS |
| Beamline | X10SA |
| Temperature [K] | 100 |
| Detector technology | CCD |
| Collection date | 2007-04-16 |
| Detector | MARMOSAIC 225 mm CCD |
| Wavelength(s) | 0.95362 |
| Spacegroup name | P 21 21 2 |
| Unit cell lengths | 120.618, 130.142, 62.477 |
| Unit cell angles | 90.00, 90.00, 90.00 |
Refinement procedure
| Resolution | 50.000 - 2.250 |
| R-factor | 0.191 |
| Rwork | 0.188 |
| R-free | 0.24400 |
| Structure solution method | MOLECULAR REPLACEMENT |
| Starting model (for MR) | assembly of PDB entries 1TX4 1ow3 1rgp 2ngr 1grn 1f7c 1am4 |
| RMSD bond length | 0.014 |
| RMSD bond angle | 1.379 |
| Data reduction software | MOSFLM |
| Data scaling software | SCALA |
| Phasing software | PHASER |
| Refinement software | REFMAC |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 50.000 | 2.370 |
| High resolution limit [Å] | 2.250 | 2.250 |
| Rmerge | 0.086 | 0.823 |
| Number of reflections | 47518 | |
| <I/σ(I)> | 15.4 | 0.9 |
| Completeness [%] | 100.0 | 99.9 |
| Redundancy | 7.1 | 6.9 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, SITTING DROP | 7.4 | 293 | 2.4M Na malate, pH 7.4, VAPOR DIFFUSION, SITTING DROP, temperature 293K |
