3BY9
Crystal structure of the V. cholerae Histidine Kinase DctB Sensor Domain
Experimental procedure
Experimental method | MAD |
Source type | SYNCHROTRON |
Source details | NSLS BEAMLINE X4A |
Synchrotron site | NSLS |
Beamline | X4A |
Temperature [K] | 100 |
Detector technology | CCD |
Collection date | 2003-02-19 |
Wavelength(s) | 0.9637, 0.9788, 0.9791, 0.9946 |
Spacegroup name | P 21 21 21 |
Unit cell lengths | 56.311, 90.982, 114.196 |
Unit cell angles | 90.00, 90.00, 90.00 |
Refinement procedure
Resolution | 19.780 - 1.700 |
Rwork | 0.162 |
R-free | 0.19900 |
Structure solution method | MAD |
RMSD bond length | 0.015 |
RMSD bond angle | 1.700 |
Data reduction software | DENZO |
Data scaling software | SCALEPACK |
Phasing software | SOLVE |
Refinement software | CNS (1.1) |
Data quality characteristics
Overall | Inner shell | Outer shell | |
Low resolution limit [Å] | 20.000 | 20.000 | 1.660 |
High resolution limit [Å] | 1.600 | 3.440 | 1.600 |
Rmerge | 0.057 | 0.032 | 0.395 |
Number of reflections | 76821 | ||
<I/σ(I)> | 11.5 | ||
Completeness [%] | 98.4 | 98.6 | 91.8 |
Crystallization Conditions
crystal ID | method | pH | temperature | details |
1 | VAPOR DIFFUSION | 7.5 | 277 | isopropanol, calcium acetate, pH 7.5, vapor diffusion, temperature 277K |