3BY8
Crystal Structure of the E.coli DcuS Sensor Domain
Experimental procedure
Experimental method | MAD |
Source type | SYNCHROTRON |
Source details | NSLS BEAMLINE X4A |
Synchrotron site | NSLS |
Beamline | X4A |
Temperature [K] | 100 |
Detector technology | CCD |
Collection date | 2001-04-07 |
Wavelength(s) | 0.9678, 0.9787, 0.9793, 0.9918 |
Spacegroup name | P 31 2 1 |
Unit cell lengths | 86.111, 86.111, 35.207 |
Unit cell angles | 90.00, 90.00, 120.00 |
Refinement procedure
Resolution | 28.190 - 1.450 |
Rwork | 0.219 |
R-free | 0.23200 |
Structure solution method | MAD |
RMSD bond length | 0.010 |
RMSD bond angle | 1.600 |
Data reduction software | DENZO |
Data scaling software | SCALEPACK |
Phasing software | DM (4.0) |
Refinement software | CNS (1.1) |
Data quality characteristics
Overall | Inner shell | Outer shell | |
Low resolution limit [Å] | 30.000 | 30.000 | 1.450 |
High resolution limit [Å] | 1.400 | 3.020 | 1.400 |
Rmerge | 0.046 | 0.041 | 0.238 |
Number of reflections | 29824 | ||
<I/σ(I)> | 17 | ||
Completeness [%] | 99.9 | 100 | 98.9 |
Crystallization Conditions
crystal ID | method | pH | temperature | details |
1 | VAPOR DIFFUSION | 4.5 | 277 | PEG MME 2000, isopropanol, ammonium citrate, pH 4.5, vapor diffusion, temperature 277K |