3BY6
Crystal structure of a transcriptional regulator from Oenococcus oeni
Experimental procedure
| Experimental method | MAD |
| Source type | SYNCHROTRON |
| Source details | APS BEAMLINE 19-ID |
| Synchrotron site | APS |
| Beamline | 19-ID |
| Temperature [K] | 100 |
| Detector technology | CCD |
| Collection date | 2007-12-03 |
| Detector | ADSC QUANTUM 315 |
| Wavelength(s) | 0.9794, 0.9796 |
| Spacegroup name | C 1 2 1 |
| Unit cell lengths | 83.434, 103.893, 131.393 |
| Unit cell angles | 90.00, 102.85, 90.00 |
Refinement procedure
| Resolution | 41.780 - 2.200 |
| R-factor | 0.24076 |
| Rwork | 0.239 |
| R-free | 0.27786 |
| Structure solution method | MAD |
| RMSD bond length | 0.016 |
| RMSD bond angle | 1.515 |
| Data reduction software | HKL-3000 |
| Data scaling software | HKL-3000 |
| Phasing software | HKL-3000 |
| Refinement software | REFMAC (5.2.0019) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 64.020 | 2.257 |
| High resolution limit [Å] | 2.200 | 2.200 |
| Rmerge | 0.073 | 0.309 |
| Number of reflections | 51521 | |
| <I/σ(I)> | 17.48 | 4.26 |
| Completeness [%] | 97.8 | 87.17 |
| Redundancy | 5 | 4.8 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, SITTING DROP | 7 | 289 | 0.2M NaCl, 0.1M Tris-HCl, 30% PEG 3000, pH 7.0, VAPOR DIFFUSION, SITTING DROP, temperature 289K |
