3BY5
Crystal structure of cobalamin biosynthesis protein chiG from Agrobacterium tumefaciens str. C58
Experimental procedure
Experimental method | SINGLE WAVELENGTH |
Source type | SYNCHROTRON |
Source details | APS BEAMLINE 31-ID |
Synchrotron site | APS |
Beamline | 31-ID |
Temperature [K] | 77 |
Detector technology | CCD |
Collection date | 2007-07-19 |
Detector | MAR CCD 165 mm |
Wavelength(s) | 0.9796 |
Spacegroup name | H 3 2 |
Unit cell lengths | 72.023, 72.023, 141.069 |
Unit cell angles | 90.00, 90.00, 120.00 |
Refinement procedure
Resolution | 20.000 - 2.520 |
R-factor | 0.21289 |
Rwork | 0.211 |
R-free | 0.27187 |
Structure solution method | SAD |
RMSD bond length | 0.007 |
RMSD bond angle | 1.132 |
Data reduction software | HKL-2000 |
Data scaling software | HKL-2000 |
Phasing software | SHELXD |
Refinement software | REFMAC (5.3.0034) |
Data quality characteristics
Overall | Outer shell | |
Low resolution limit [Å] | 50.000 | 2.610 |
High resolution limit [Å] | 2.520 | 2.520 |
Rmerge | 0.138 | 0.890 |
Number of reflections | 4985 | |
<I/σ(I)> | 3.7 | 1.2 |
Completeness [%] | 99.6 | 97.2 |
Redundancy | 6.4 | 5.3 |
Crystallization Conditions
crystal ID | method | pH | temperature | details |
1 | VAPOR DIFFUSION, SITTING DROP | 5.2 | 294 | 100mM Bis-Tris pH 5.2, 29% PEG 3350, 200mM Ammonium sulfate, 10% Glycerol, VAPOR DIFFUSION, SITTING DROP, temperature 294K |