3BY4

Structure of Ovarian Tumor (OTU) domain in complex with Ubiquitin

Experimental procedure

Experimental method	MAD
Source type	SYNCHROTRON
Source details	APS BEAMLINE 19-ID
Synchrotron site	APS
Beamline	19-ID
Temperature [K]	100
Detector technology	CCD
Collection date	2005-03-25
Detector	MARRESEARCH
Wavelength(s)	1.0722, 1.7025, 1.0450
Spacegroup name	P 21 21 21
Unit cell lengths	46.304, 73.215, 88.880
Unit cell angles	90.00, 90.00, 90.00

Refinement procedure

Resolution	37.990 - 1.550
R-factor	0.191
Rwork	0.189
R-free	0.21100
Structure solution method	MAD
RMSD bond length	0.005
RMSD bond angle	0.851
Data reduction software	DENZO
Data scaling software	SCALEPACK
Phasing software	SHARP
Refinement software	PHENIX

Data quality characteristics

	Overall
Low resolution limit [Å]	37.990
High resolution limit [Å]	1.550
Rmerge	0.037
Number of reflections	44240
<I/σ(I)>	31.2
Completeness [%]	99.2
Redundancy	6.6

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	VAPOR DIFFUSION, HANGING DROP	6.5	277	Bis-Tris, Magnesium Chloride, PEG 3350, pH 6.5, VAPOR DIFFUSION, HANGING DROP, temperature 277K
1	VAPOR DIFFUSION, HANGING DROP	6.5	277	Bis-Tris, Magnesium Chloride, PEG 3350, pH 6.5, VAPOR DIFFUSION, HANGING DROP, temperature 277K