3BXZ
Crystal structure of the isolated DEAD motor domains from Escherichia coli SecA
Experimental procedure
Experimental method | SINGLE WAVELENGTH |
Source type | SYNCHROTRON |
Source details | APS BEAMLINE 19-ID |
Synchrotron site | APS |
Beamline | 19-ID |
Temperature [K] | 100 |
Detector technology | CCD |
Collection date | 2006-10-28 |
Detector | ADSC QUANTUM 315 |
Wavelength(s) | 0.97902 |
Spacegroup name | C 1 2 1 |
Unit cell lengths | 177.823, 71.361, 119.536 |
Unit cell angles | 90.00, 128.82, 90.00 |
Refinement procedure
Resolution | 46.575 - 3.000 |
R-factor | 0.245 |
Rwork | 0.245 |
R-free | 0.30500 |
Structure solution method | MOLECULAR REPLACEMENT |
Starting model (for MR) | 2fsi |
Data reduction software | MOSFLM |
Data scaling software | SCALA |
Phasing software | MOLREP |
Refinement software | CNS |
Data quality characteristics
Overall | Outer shell | |
Low resolution limit [Å] | 46.575 | 3.160 |
High resolution limit [Å] | 3.000 | 3.000 |
Rmerge | 0.069 | 0.451 |
Total number of observations | 12284 | |
Number of reflections | 23500 | |
<I/σ(I)> | 8.2 | 1.7 |
Completeness [%] | 99.5 | 100 |
Redundancy | 3.5 | 3.6 |
Crystallization Conditions
crystal ID | method | pH | temperature | details |
1 | VAPOR DIFFUSION, SITTING DROP | 7.5 | 291 | 25% Ethylene glycol, 0.025M Spermidine, pH 7.5, VAPOR DIFFUSION, SITTING DROP, temperature 291K |