3BXU
PpcB, A Cytochrome c7 from Geobacter sulfurreducens
Experimental procedure
| Experimental method | MAD |
| Source type | SYNCHROTRON |
| Source details | APS BEAMLINE 19-BM |
| Synchrotron site | APS |
| Beamline | 19-BM |
| Temperature [K] | 100 |
| Detector technology | CCD |
| Collection date | 2003-12-18 |
| Detector | CUSTOM-MADE |
| Wavelength(s) | 1.73766, 1.73859, 1.78875 |
| Spacegroup name | P 21 21 21 |
| Unit cell lengths | 34.200, 47.500, 88.900 |
| Unit cell angles | 90.00, 90.00, 90.00 |
Refinement procedure
| Resolution | 19.070 - 1.350 |
| R-factor | 0.16411 |
| Rwork | 0.162 |
| R-free | 0.18575 |
| Structure solution method | MAD |
| RMSD bond length | 0.016 |
| RMSD bond angle | 1.648 |
| Data reduction software | HKL-2000 |
| Data scaling software | HKL-2000 |
| Phasing software | CNS |
| Refinement software | REFMAC (5.1.24) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 50.000 | 1.400 |
| High resolution limit [Å] | 1.350 | 1.350 |
| Rmerge | 0.089 | 0.342 |
| Number of reflections | 58322 | |
| <I/σ(I)> | 22.4 | 2.5 |
| Completeness [%] | 93.0 | 62 |
| Redundancy | 3.3 | 1.6 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, HANGING DROP | 7.8 | 298 | 45% Jeffamine ED-2001 0.2 M ammonium iodide, pH 7.8, VAPOR DIFFUSION, HANGING DROP, temperature 298K |
