3BXA
Monomeric Far-red Fluorescent Protein mKate Crystallized at pH 4.2
Experimental procedure
Experimental method | SINGLE WAVELENGTH |
Source type | SYNCHROTRON |
Source details | APS BEAMLINE 22-ID |
Synchrotron site | APS |
Beamline | 22-ID |
Temperature [K] | 100 |
Detector technology | CCD |
Detector | MARMOSAIC 300 mm CCD |
Wavelength(s) | 1.0 |
Spacegroup name | I 4 |
Unit cell lengths | 98.181, 98.181, 106.452 |
Unit cell angles | 90.00, 90.00, 90.00 |
Refinement procedure
Resolution | 29.070 - 1.750 |
R-factor | 0.182 |
Rwork | 0.180 |
R-free | 0.23300 |
RMSD bond length | 0.015 |
RMSD bond angle | 1.766 |
Data reduction software | DENZO |
Data scaling software | SCALEPACK |
Phasing software | MOLREP |
Refinement software | REFMAC |
Data quality characteristics
Overall | Inner shell | Outer shell | |
Low resolution limit [Å] | 30.000 | 30.000 | 1.810 |
High resolution limit [Å] | 1.750 | 3.770 | 1.750 |
Rmerge | 0.041 | 0.020 | 0.522 |
Number of reflections | 50567 | ||
<I/σ(I)> | 13.4 | ||
Completeness [%] | 99.6 | 99.9 | 99.1 |
Redundancy | 7.7 | 7.7 | 7.6 |
Crystallization Conditions
crystal ID | method | pH | temperature | details |
1 | VAPOR DIFFUSION, HANGING DROP | 4.2 | 293 | 17.5% w/v PEG 3350, 0.07M citric acid, pH 4.2, VAPOR DIFFUSION, HANGING DROP, temperature 293K |