3BX8
Engineered Human Lipocalin 2 (LCN2), apo-form
Experimental procedure
Experimental method | SINGLE WAVELENGTH |
Source type | SYNCHROTRON |
Source details | BESSY BEAMLINE 14.1 |
Synchrotron site | BESSY |
Beamline | 14.1 |
Temperature [K] | 100 |
Detector technology | CCD |
Collection date | 2006-10-31 |
Detector | MARMOSAIC 225 mm CCD |
Wavelength(s) | 0.95373 |
Spacegroup name | P 1 |
Unit cell lengths | 61.482, 92.861, 98.825 |
Unit cell angles | 110.77, 90.00, 109.36 |
Refinement procedure
Resolution | 20.000 - 2.000 |
Rwork | 0.251 |
R-free | 0.28200 |
Starting model (for MR) | 1DFV chain B |
Data reduction software | HKL-2000 |
Data scaling software | HKL-2000 |
Phasing software | PHASER |
Refinement software | CNS |
Data quality characteristics
Overall | Outer shell | |
Low resolution limit [Å] | 20.000 | 2.070 |
High resolution limit [Å] | 2.000 | 2.000 |
Number of reflections | 129807 | |
<I/σ(I)> | 16.2 | 2 |
Completeness [%] | 83.2 | 46.9 |
Redundancy | 1.9 | 1.6 |
Crystallization Conditions
crystal ID | method | pH | temperature | details |
1 | VAPOR DIFFUSION, SITTING DROP | 5.45 | 293 | 1.45M ammonium sulphate, 0.1M Na-cacodylate, 0.1% (v/v) Anapoe X-405, pH 5.45, VAPOR DIFFUSION, SITTING DROP, temperature 293K |