3BWU
Crystal structure of the ternary complex of FimD (N-Terminal Domain, FimDN) with FimC and the N-terminally truncated pilus subunit FimF (FimFt)
Experimental procedure
Experimental method | SINGLE WAVELENGTH |
Source type | SYNCHROTRON |
Source details | SLS BEAMLINE X06SA |
Synchrotron site | SLS |
Beamline | X06SA |
Temperature [K] | 90 |
Detector technology | CCD |
Collection date | 2006-04-28 |
Detector | MARRESEARCH |
Wavelength(s) | 0.88562 |
Spacegroup name | P 31 2 1 |
Unit cell lengths | 126.948, 126.948, 68.755 |
Unit cell angles | 90.00, 90.00, 120.00 |
Refinement procedure
Resolution | 32.340 - 1.760 |
Rwork | 0.175 |
R-free | 0.21200 |
Starting model (for MR) | 1ze3 |
RMSD bond length | 0.003 |
RMSD bond angle | 0.635 |
Data reduction software | DENZO |
Data scaling software | SCALEPACK |
Phasing software | PHASER |
Refinement software | PHENIX |
Data quality characteristics
Overall | Inner shell | Outer shell | |
Low resolution limit [Å] | 33.000 | 33.000 | 1.820 |
High resolution limit [Å] | 1.760 | 3.790 | 1.760 |
Rmerge | 0.081 | 0.044 | 0.354 |
Number of reflections | 63367 | ||
<I/σ(I)> | 8.6 | ||
Completeness [%] | 99.9 | 100 | 99.3 |
Redundancy | 5.4 | 5.5 | 4.4 |
Crystallization Conditions
crystal ID | method | pH | temperature | details |
1 | VAPOR DIFFUSION, SITTING DROP | 277 | 20 mM Tris/HCl pH 8.0, 20% PEG 8000, 50 mM succinic acid pH 4.0, VAPOR DIFFUSION, SITTING DROP, temperature 277K |