3BWH
Atomic resolution structure of cucurmosin, a novel type 1 RIP from the sarcocarp of Cucurbita moschata
Experimental procedure
Experimental method | SINGLE WAVELENGTH |
Source type | SYNCHROTRON |
Source details | APS BEAMLINE 22-ID |
Synchrotron site | APS |
Beamline | 22-ID |
Temperature [K] | 93 |
Detector technology | CCD |
Collection date | 2002-11-01 |
Detector | MARMOSAIC 325 mm CCD |
Spacegroup name | P 21 21 21 |
Unit cell lengths | 41.763, 58.782, 99.523 |
Unit cell angles | 90.00, 90.00, 90.00 |
Refinement procedure
Resolution | 38.520 - 1.000 |
R-factor | 0.172 |
Rwork | 0.171 |
R-free | 0.17900 |
Structure solution method | MOLECULAR REPLACEMENT |
Starting model (for MR) | 1tcs |
RMSD bond length | 0.008 |
RMSD bond angle | 1.498 |
Data reduction software | HKL-2000 |
Data scaling software | HKL-2000 |
Phasing software | AMoRE |
Refinement software | REFMAC (5.2.0019) |
Data quality characteristics
Overall | Outer shell | |
Low resolution limit [Å] | 50.000 | 1.040 |
High resolution limit [Å] | 1.000 | 1.000 |
Rmerge | 0.108 | 0.393 |
Number of reflections | 111623 | |
<I/σ(I)> | 22.9 | 1.3 |
Completeness [%] | 84.0 | 40.6 |
Redundancy | 7.4 | 2.1 |
Crystallization Conditions
crystal ID | method | pH | temperature | details |
1 | 5.9 | 298 | 100 mM phosphate buffer, 20% PEG6000, pH 5.9, VAPOR DIFFUSION, HANGING DROP, temperature 298K, pH 5.90 |