3BWG
The crystal structure of possible transcriptional regulator YydK from Bacillus subtilis subsp. subtilis str. 168
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | APS BEAMLINE 19-ID |
| Synchrotron site | APS |
| Beamline | 19-ID |
| Temperature [K] | 100 |
| Detector technology | CCD |
| Collection date | 2007-03-05 |
| Detector | ADSC QUANTUM 315 |
| Wavelength(s) | 0.97918 |
| Spacegroup name | C 1 2 1 |
| Unit cell lengths | 101.994, 58.284, 138.823 |
| Unit cell angles | 90.00, 110.08, 90.00 |
Refinement procedure
| Resolution | 43.980 - 2.090 |
| R-factor | 0.18851 |
| Rwork | 0.186 |
| R-free | 0.22846 |
| Structure solution method | SAD |
| RMSD bond length | 0.016 |
| RMSD bond angle | 1.677 |
| Data reduction software | HKL-3000 |
| Data scaling software | HKL-3000 |
| Phasing software | SHELXD |
| Refinement software | REFMAC (5.2.0019) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 43.980 | 2.180 |
| High resolution limit [Å] | 2.090 | 2.090 |
| Rmerge | 0.070 | 0.440 |
| Number of reflections | 40580 | |
| <I/σ(I)> | 23.8 | 1.65 |
| Completeness [%] | 89.5 | 43.3 |
| Redundancy | 3.3 | 2.2 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, SITTING DROP | 5.5 | 289 | 0.1M Ammonium acetate, 0.1M Bis-Tris, 17% PEG 10000, pH 5.5, VAPOR DIFFUSION, SITTING DROP, temperature 289K |
