3BWF
Crystal structure of the human Pim1 in complex with an osmium compound
Experimental procedure
Experimental method | SINGLE WAVELENGTH |
Source type | SYNCHROTRON |
Source details | NSLS BEAMLINE X12C |
Synchrotron site | NSLS |
Beamline | X12C |
Detector technology | CCD |
Collection date | 2006-07-20 |
Detector | ADSC QUANTUM 210 |
Spacegroup name | P 65 |
Unit cell lengths | 98.458, 98.458, 81.362 |
Unit cell angles | 90.00, 90.00, 120.00 |
Refinement procedure
Resolution | 42.640 - 2.350 |
R-factor | 0.1707 |
Rwork | 0.168 |
R-free | 0.22542 |
Structure solution method | MOLECULAR REPLACEMENT |
RMSD bond length | 0.016 |
RMSD bond angle | 1.447 |
Data reduction software | HKL-2000 |
Data scaling software | HKL-2000 |
Phasing software | AMoRE |
Refinement software | REFMAC (5.4.0066) |
Data quality characteristics
Overall | Outer shell | |
Low resolution limit [Å] | 50.000 | 2.430 |
High resolution limit [Å] | 2.350 | 2.350 |
Rmerge | 0.149 | 0.789 |
Number of reflections | 18397 | |
<I/σ(I)> | 18.1 | 3.5 |
Completeness [%] | 97.7 | 100 |
Redundancy | 11.5 | 11.5 |
Crystallization Conditions
crystal ID | method | pH | temperature | details |
1 | VAPOR DIFFUSION, SITTING DROP | 7 | 277 | 0.2 M lithium sulfate, 100 mM BisTrisPropane, 20% PEG3350, 10% ethylene glycol, 0.3% DMSO, pH 7.0, VAPOR DIFFUSION, SITTING DROP, temperature 277K |