3BWB
Crystal structure of the apo form of spermidine synthase from Trypanosoma cruzi at 2.5 A resolution
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | ALS BEAMLINE 8.2.2 |
| Synchrotron site | ALS |
| Beamline | 8.2.2 |
| Temperature [K] | 100 |
| Detector technology | CCD |
| Collection date | 2005-06-25 |
| Detector | ADSC QUANTUM 315 |
| Wavelength(s) | 0.97000 |
| Spacegroup name | P 1 21 1 |
| Unit cell lengths | 43.669, 96.996, 69.649 |
| Unit cell angles | 90.00, 106.33, 90.00 |
Refinement procedure
| Resolution | 20.000 - 2.500 |
| R-factor | 0.20068 |
| Rwork | 0.198 |
| R-free | 0.24785 |
| Structure solution method | SAD |
| RMSD bond length | 0.007 |
| RMSD bond angle | 1.058 |
| Data reduction software | XDS |
| Data scaling software | XSCALE |
| Phasing software | SHELXD |
| Refinement software | REFMAC (5.2.0019) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 20.000 | 2.600 |
| High resolution limit [Å] | 2.500 | 2.500 |
| Rmerge | 0.070 | 0.396 |
| Number of reflections | 16956 | |
| <I/σ(I)> | 12.23 | 3.2 |
| Completeness [%] | 87.5 | 53.6 |
| Redundancy | 3.3 | 2.1 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, SITTING DROP | 8 | 277 | 40 % PEG 4000, 6% PEG 20000, 0.1 M Tris-HCl, 0.1 M Potassium phosphate, pH 8.0, VAPOR DIFFUSION, SITTING DROP, temperature 277K |
