3BW8
Crystal structure of the Clostridium limosum C3 exoenzyme
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | SLS BEAMLINE X06SA |
| Synchrotron site | SLS |
| Beamline | X06SA |
| Temperature [K] | 100 |
| Detector technology | CCD |
| Collection date | 2004-10-07 |
| Detector | MARRESEARCH |
| Wavelength(s) | 0.9793 |
| Spacegroup name | P 1 21 1 |
| Unit cell lengths | 40.000, 91.540, 70.850 |
| Unit cell angles | 90.00, 106.45, 90.00 |
Refinement procedure
| Resolution | 11.800 - 1.800 |
| R-factor | 0.1969 |
| Rwork | 0.195 |
| R-free | 0.23360 |
| Structure solution method | MOLECULAR REPLACEMENT |
| Starting model (for MR) | 1g24 |
| RMSD bond length | 0.010 |
| RMSD bond angle | 1.196 |
| Data reduction software | XDS |
| Data scaling software | XSCALE |
| Phasing software | MOLREP |
| Refinement software | TNT |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 30.000 | 1.900 |
| High resolution limit [Å] | 1.800 | 1.800 |
| Rmerge | 0.082 | 0.360 |
| Number of reflections | 43029 | |
| <I/σ(I)> | 6.11 | 2.3 |
| Completeness [%] | 90.4 | 72.2 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, SITTING DROP | 7.5 | 293 | 30% PEG 8000, 200 MM (NH4)2SO4, pH 7.5, VAPOR DIFFUSION, SITTING DROP, temperature 293K |
