3BW6

Crystal structure of the longin domain of yeast Ykt6

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	SYNCHROTRON
Source details	SLS BEAMLINE X10SA
Synchrotron site	SLS
Beamline	X10SA
Temperature [K]	100
Detector technology	CCD
Detector	MARMOSAIC 225 mm CCD
Wavelength(s)	0.934
Spacegroup name	P 32 2 1
Unit cell lengths	78.890, 78.890, 64.660
Unit cell angles	90.00, 90.00, 120.00

Refinement procedure

Resolution	20.000 - 2.500
R-factor	0.24
Rwork	0.240
R-free	0.26000
Structure solution method	SAD
RMSD bond length	0.009
RMSD bond angle	1.279
Data reduction software	XDS
Data scaling software	XDS
Phasing software	CNS
Refinement software	CNS

Data quality characteristics

	Overall	Outer shell
Low resolution limit [Å]	20.000	2.550
High resolution limit [Å]	2.500	2.500
Number of reflections	8293
Completeness [%]	94.4	100

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	VAPOR DIFFUSION, HANGING DROP	6.5	293	26% PEG 3350, 150 mM ammonium sulphate, 100 mM MES (pH 6.5), VAPOR DIFFUSION, HANGING DROP, temperature 293K