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3BW1

Crystal structure of homomeric yeast Lsm3 exhibiting novel octameric ring organisation

Experimental procedure
Experimental methodSINGLE WAVELENGTH
Source typeSYNCHROTRON
Source detailsAPS BEAMLINE 23-ID-D
Synchrotron siteAPS
Beamline23-ID-D
Temperature [K]100
Detector technologyCCD
Collection date2005-11-25
DetectorMARMOSAIC 300 mm CCD
Wavelength(s)0.9793
Spacegroup nameI 4 2 2
Unit cell lengths83.743, 83.743, 141.931
Unit cell angles90.00, 90.00, 90.00
Refinement procedure
Resolution24.040 - 2.500
R-factor0.21694
Rwork0.216
R-free0.24296
Structure solution methodMOLECULAR REPLACEMENT
Starting model (for MR)1i81
RMSD bond length0.021
RMSD bond angle2.125
Data reduction softwareMOSFLM
Data scaling softwareSCALA
Phasing softwarePHASER
Refinement softwareREFMAC (5.2.0019)
Data quality characteristics
 OverallOuter shell
Low resolution limit [Å]36.2502.570
High resolution limit [Å]2.4502.450
Rmerge0.0861.405
Number of reflections90731169
<I/σ(I)>23.71.5
Completeness [%]97.683.9
Crystallization Conditions
crystal IDmethodpHtemperaturedetails
1VAPOR DIFFUSION, HANGING DROP72931.75M lithium sulfate, 8% (v/v) 2-methyl-2,4-pentanediol, 0.1M imidazole (pH 7.0), 10mM uridine-5-monophosphate, VAPOR DIFFUSION, HANGING DROP, temperature 293K

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