3BVQ

Crystal Structure of Apo NotI Restriction Endonuclease

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	SYNCHROTRON
Source details	ALS BEAMLINE 5.0.2
Synchrotron site	ALS
Beamline	5.0.2
Wavelength(s)	0.9804, 0.9805, 0.9744, 1.0048
Spacegroup name	P 43 21 2
Unit cell lengths	74.893, 74.893, 274.110
Unit cell angles	90.00, 90.00, 90.00

Refinement procedure

Resolution	45.830 - 2.800
R-factor	0.266
Rwork	0.262
R-free	0.33500
RMSD bond length	0.007
RMSD bond angle	1.103
Data reduction software	DENZO
Data scaling software	SCALEPACK
Phasing software	SHARP
Refinement software	REFMAC (5.2.0019)

Data quality characteristics

	Overall	Outer shell
Low resolution limit [Å]	50.000	2.900
High resolution limit [Å]	2.800	2.800
Rmerge	0.109	0.317
Number of reflections	20201
<I/σ(I)>	11.2
Completeness [%]	99.6	97.1
Redundancy	26	21

Crystallization Conditions

crystal ID	method	pH	temperature	details
1