3BVP
Crystal Structure of the N-terminal Catalytic Domain of TP901-1 Integrase
Experimental procedure
Experimental method | SINGLE WAVELENGTH |
Source type | SYNCHROTRON |
Source details | ALS BEAMLINE 8.2.1 |
Synchrotron site | ALS |
Beamline | 8.2.1 |
Temperature [K] | 100 |
Detector technology | CCD |
Collection date | 2007-07-08 |
Detector | ADSC QUANTUM 4 |
Wavelength(s) | 1.0093 |
Spacegroup name | P 41 21 2 |
Unit cell lengths | 67.229, 67.229, 174.565 |
Unit cell angles | 90.00, 90.00, 90.00 |
Refinement procedure
Resolution | 44.000 - 2.100 |
R-factor | 0.201 |
Rwork | 0.198 |
R-free | 0.26300 |
Structure solution method | MAD |
RMSD bond length | 0.011 |
RMSD bond angle | 1.212 |
Data reduction software | DENZO |
Data scaling software | SCALEPACK |
Phasing software | SOLVE (2.03) |
Refinement software | REFMAC |
Data quality characteristics
Overall | Inner shell | Outer shell | |
Low resolution limit [Å] | 50.000 | 50.000 | 2.180 |
High resolution limit [Å] | 2.100 | 4.520 | 2.100 |
Rmerge | 0.056 | 0.026 | 0.440 |
Number of reflections | 23563 | ||
<I/σ(I)> | 15.8 | ||
Completeness [%] | 96.4 | 97.9 | 77.8 |
Redundancy | 8.1 | 8 | 5.1 |
Crystallization Conditions
crystal ID | method | pH | temperature | details |
1 | VAPOR DIFFUSION, HANGING DROP | 6 | 294 | PEG8000, Mg(OAc)2, pH 6.0, vapor diffusion, hanging drop, temperature 294K |
1 | VAPOR DIFFUSION, HANGING DROP | 6 | 294 | PEG8000, Mg(OAc)2, pH 6.0, vapor diffusion, hanging drop, temperature 294K |
1 | VAPOR DIFFUSION, HANGING DROP | 6 | 294 | PEG8000, Mg(OAc)2, pH 6.0, vapor diffusion, hanging drop, temperature 294K |