3BVC
Crystal structure of uncharacterized protein Ism_01780 from Roseovarius nubinhibens ISM
Experimental procedure
Experimental method | SINGLE WAVELENGTH |
Source type | SYNCHROTRON |
Source details | APS BEAMLINE 31-ID |
Synchrotron site | APS |
Beamline | 31-ID |
Temperature [K] | 77 |
Detector technology | CCD |
Collection date | 2007-12-05 |
Detector | MAR CCD 165 mm |
Wavelength(s) | 0.9796 |
Spacegroup name | P 21 21 2 |
Unit cell lengths | 96.405, 101.973, 76.506 |
Unit cell angles | 90.00, 90.00, 90.00 |
Refinement procedure
Resolution | 20.000 - 2.750 |
R-factor | 0.25668 |
Rwork | 0.255 |
R-free | 0.29796 |
Structure solution method | SAD |
RMSD bond length | 0.006 |
RMSD bond angle | 1.024 |
Data reduction software | HKL-2000 |
Data scaling software | HKL-2000 |
Phasing software | SHELXD |
Refinement software | REFMAC (5.3.0034) |
Data quality characteristics
Overall | Outer shell | |
Low resolution limit [Å] | 50.000 | 2.850 |
High resolution limit [Å] | 2.750 | 2.750 |
Rmerge | 0.140 | 0.860 |
Number of reflections | 20512 | |
<I/σ(I)> | 3.2 | 0.45 |
Completeness [%] | 90.7 | 98.5 |
Redundancy | 3.2 | 2.9 |
Crystallization Conditions
crystal ID | method | pH | temperature | details |
1 | VAPOR DIFFUSION, SITTING DROP | 7.5 | 294 | 0.1M HEPES, 0.2M Calcium chloride, 28% PEG 400, pH 7.5, VAPOR DIFFUSION, SITTING DROP, temperature 294K |