3BV6
Crystal structure of uncharacterized metallo protein from Vibrio cholerae with beta-lactamase like fold
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | APS BEAMLINE 5ID-B |
| Synchrotron site | APS |
| Beamline | 5ID-B |
| Temperature [K] | 100 |
| Detector technology | CCD |
| Collection date | 2006-06-16 |
| Detector | MARMOSAIC 225 mm CCD |
| Wavelength(s) | 1.0000 |
| Spacegroup name | P 1 |
| Unit cell lengths | 83.433, 94.839, 94.591 |
| Unit cell angles | 99.69, 97.90, 116.13 |
Refinement procedure
| Resolution | 25.000 - 1.800 |
| R-factor | 0.16873 |
| Rwork | 0.167 |
| R-free | 0.20754 |
| Structure solution method | MIRAS |
| RMSD bond length | 0.015 |
| RMSD bond angle | 1.660 |
| Data reduction software | HKL-2000 |
| Data scaling software | HKL-2000 |
| Phasing software | SHARP |
| Refinement software | REFMAC (5.2.0019) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 30.000 | 1.860 |
| High resolution limit [Å] | 1.800 | 1.800 |
| Rmerge | 0.062 | 0.394 |
| Number of reflections | 224520 | |
| <I/σ(I)> | 18.6 | 3 |
| Completeness [%] | 97.4 | 94.2 |
| Redundancy | 3.5 | 3.3 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, SITTING DROP | 7.5 | 295 | 0.1M HEPES, 0.25M Sodium chloride, 25% (w/v) PEG 4000, pH 7.5, VAPOR DIFFUSION, SITTING DROP, temperature 295K |
