3BUZ
Crystal structure of ia-bTAD-actin complex
Experimental procedure
Experimental method | SINGLE WAVELENGTH |
Source type | SYNCHROTRON |
Source details | PHOTON FACTORY BEAMLINE AR-NW12A |
Synchrotron site | Photon Factory |
Beamline | AR-NW12A |
Temperature [K] | 100 |
Detector technology | CCD |
Collection date | 2006-12-05 |
Detector | ADSC QUANTUM 210 |
Wavelength(s) | 1.0 |
Spacegroup name | P 21 21 21 |
Unit cell lengths | 57.014, 126.344, 147.127 |
Unit cell angles | 90.00, 90.00, 90.00 |
Refinement procedure
Resolution | 36.690 - 2.810 |
R-factor | 0.22747 |
Rwork | 0.224 |
R-free | 0.29845 |
Structure solution method | MOLECULAR REPLACEMENT |
Starting model (for MR) | 1GIQ AND 1IJJ |
RMSD bond length | 0.023 |
RMSD bond angle | 2.198 |
Data reduction software | HKL-2000 |
Data scaling software | HKL-2000 |
Phasing software | MOLREP |
Refinement software | REFMAC (5.2.0019) |
Data quality characteristics
Overall | Outer shell | |
Low resolution limit [Å] | 36.690 | 2.900 |
High resolution limit [Å] | 2.800 | 2.800 |
Number of reflections | 26445 | |
Completeness [%] | 99.4 | 99.7 |
Crystallization Conditions
crystal ID | method | pH | temperature | details |
1 | VAPOR DIFFUSION, HANGING DROP | 6.5 | 277 | 20% PEG1000, 0.1M MES(pH6.5), VAPOR DIFFUSION, HANGING DROP, temperature 277K |