3BUU
Crystal structure of LolA superfamily protein NE2245 from Nitrosomonas europaea
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | APS BEAMLINE 19-ID |
| Synchrotron site | APS |
| Beamline | 19-ID |
| Temperature [K] | 100 |
| Detector technology | CCD |
| Collection date | 2007-06-17 |
| Detector | ADSC QUANTUM 315 |
| Wavelength(s) | 0.97940 |
| Spacegroup name | P 1 21 1 |
| Unit cell lengths | 35.019, 59.116, 89.676 |
| Unit cell angles | 90.00, 90.72, 90.00 |
Refinement procedure
| Resolution | 49.330 - 1.200 |
| R-factor | 0.16004 |
| Rwork | 0.158 |
| R-free | 0.19227 |
| Structure solution method | SAD |
| RMSD bond length | 0.015 |
| RMSD bond angle | 1.702 |
| Data reduction software | HKL-3000 |
| Data scaling software | HKL-3000 |
| Phasing software | HKL-3000 |
| Refinement software | REFMAC (5.2.0019) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 50.000 | 1.240 |
| High resolution limit [Å] | 1.200 | 1.200 |
| Rmerge | 0.093 | 0.342 |
| Number of reflections | 112699 | |
| <I/σ(I)> | 47.67 | 6.84 |
| Completeness [%] | 98.5 | 96.1 |
| Redundancy | 8.9 | 7.9 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, SITTING DROP | 7.5 | 289 | 0.1M HEPES pH 7.5, 25% PEG 1000, VAPOR DIFFUSION, SITTING DROP, temperature 289K |
