3BUL
E. coli I690C/G743C MetH C-terminal fragment (649-1227)
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | APS BEAMLINE 23-ID-B |
| Synchrotron site | APS |
| Beamline | 23-ID-B |
| Detector technology | CCD |
| Collection date | 2007-04-01 |
| Detector | MARMOSAIC 300 mm CCD |
| Wavelength(s) | 0.9793 |
| Spacegroup name | P 43 21 2 |
| Unit cell lengths | 106.529, 106.529, 137.150 |
| Unit cell angles | 90.00, 90.00, 90.00 |
Refinement procedure
| Resolution | 42.070 - 2.300 |
| R-factor | 0.2 |
| Rwork | 0.198 |
| R-free | 0.24700 |
| Structure solution method | MOLECULAR REPLACEMENT |
| Starting model (for MR) | 1k7y |
| RMSD bond length | 0.025 |
| RMSD bond angle | 2.318 |
| Data reduction software | XDS |
| Data scaling software | XSCALE |
| Phasing software | EPMR |
| Refinement software | REFMAC |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 42.070 | 2.430 |
| High resolution limit [Å] | 2.290 | 2.290 |
| Rmerge | 0.146 | 0.809 |
| Number of reflections | 35908 | 5516 |
| <I/σ(I)> | 16 | 3.46 |
| Completeness [%] | 99.2 | 95.3 |
| Redundancy | 17.306 | 15.01 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | EVAPORATION | 7.2 | 310 | 0.2 M potassium nitrate, and 20 % (w/v) PEG3350, pH 7.2, EVAPORATION, temperature 310K |
