3BU8
Crystal Structure of TRF2 TRFH domain and TIN2 peptide complex
Experimental procedure
Experimental method | SINGLE WAVELENGTH |
Source type | SYNCHROTRON |
Source details | APS BEAMLINE 23-ID-B |
Synchrotron site | APS |
Beamline | 23-ID-B |
Detector technology | CCD |
Collection date | 2006-12-06 |
Detector | MARMOSAIC 300 mm CCD |
Wavelength(s) | 1.03320 |
Spacegroup name | P 41 21 2 |
Unit cell lengths | 75.235, 75.235, 181.600 |
Unit cell angles | 90.00, 90.00, 90.00 |
Refinement procedure
Resolution | 500.000 - 2.150 |
Rwork | 0.238 |
R-free | 0.25640 |
Structure solution method | MOLECULAR REPLACEMENT |
Starting model (for MR) | 1h6p |
RMSD bond length | 0.007 |
RMSD bond angle | 1.568 |
Data reduction software | HKL-2000 |
Data scaling software | HKL-2000 |
Phasing software | PHASER |
Refinement software | CNS (1.1) |
Data quality characteristics
Overall | Outer shell | |
Low resolution limit [Å] | 500.000 | 2.230 |
High resolution limit [Å] | 2.150 | 2.150 |
Rmerge | 0.060 | 0.437 |
Number of reflections | 28753 | |
<I/σ(I)> | 41.78 | 5.63 |
Completeness [%] | 99.4 | 99.9 |
Redundancy | 13.6 | 11.4 |
Crystallization Conditions
crystal ID | method | pH | temperature | details |
1 | VAPOR DIFFUSION, HANGING DROP | 8 | 289 | PEG550 MME 14%, HEPES.Na 50 mM Tris.HCl 40 mM, MgCl2 5 mM, DTT 2 mM, pH 8.0, VAPOR DIFFUSION, HANGING DROP, temperature 289K |