3BTR
AR-NLS:Importin-alpha complex
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | ROTATING ANODE |
| Source details | RIGAKU RUH3R |
| Temperature [K] | 100 |
| Detector technology | IMAGE PLATE |
| Collection date | 2005-12-28 |
| Detector | MAR scanner 345 mm plate |
| Wavelength(s) | 1.5418 |
| Spacegroup name | P 21 21 21 |
| Unit cell lengths | 78.274, 89.372, 98.104 |
| Unit cell angles | 90.00, 90.00, 90.00 |
Refinement procedure
| Resolution | 20.110 - 2.600 |
| R-factor | 0.18859 |
| Rwork | 0.186 |
| R-free | 0.22890 |
| Structure solution method | MOLECULAR REPLACEMENT |
| Starting model (for MR) | 1ejy |
| RMSD bond length | 0.012 |
| RMSD bond angle | 1.293 |
| Data reduction software | MOSFLM |
| Data scaling software | SCALA |
| Phasing software | REFMAC (5.2.001) |
| Refinement software | REFMAC |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 21.393 | 2.740 |
| High resolution limit [Å] | 2.576 | 2.600 |
| Rmerge | 0.066 | 0.262 |
| Number of reflections | 21477 | |
| <I/σ(I)> | 8 | 2.9 |
| Completeness [%] | 98.0 | 88.1 |
| Redundancy | 5 | 4.6 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, HANGING DROP | 6.5 | 291 | pH 6.5, VAPOR DIFFUSION, HANGING DROP, temperature 291K |
