3BT5
Crystal structure of DUF305 fragment from Deinococcus radiodurans
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | APS BEAMLINE 31-ID |
| Synchrotron site | APS |
| Beamline | 31-ID |
| Temperature [K] | 100 |
| Detector technology | CCD |
| Collection date | 2007-12-09 |
| Detector | MAR CCD 165 mm |
| Wavelength(s) | 0.97930 |
| Spacegroup name | P 41 |
| Unit cell lengths | 49.690, 49.690, 71.191 |
| Unit cell angles | 90.00, 90.00, 90.00 |
Refinement procedure
| Resolution | 25.010 - 1.350 |
| R-factor | 0.156 |
| Rwork | 0.154 |
| R-free | 0.18800 |
| Structure solution method | MOLECULAR REPLACEMENT |
| Starting model (for MR) | 2qf9 |
| RMSD bond length | 0.011 |
| RMSD bond angle | 1.328 |
| Data reduction software | DENZO |
| Data scaling software | SCALEPACK |
| Phasing software | MOLREP |
| Refinement software | REFMAC |
Data quality characteristics
| Overall | Inner shell | Outer shell | |
| Low resolution limit [Å] | 50.000 | 50.000 | 1.400 |
| High resolution limit [Å] | 1.350 | 2.910 | 1.350 |
| Rmerge | 0.042 | 0.031 | 0.655 |
| Number of reflections | 37848 | ||
| <I/σ(I)> | 9.9 | ||
| Completeness [%] | 99.7 | 97 | 100 |
| Redundancy | 7.2 | 7 | 7 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, SITTING DROP | 5.5 | 298 | 0.1M Bis-Tris pH 5.5, 3M Sodium chloride, VAPOR DIFFUSION, SITTING DROP, temperature 298K |
