3BT4
Crystal Structure Analysis of AmFPI-1, fungal protease inhibitor from Antheraea mylitta
Experimental procedure
Experimental method | SINGLE WAVELENGTH |
Source type | ROTATING ANODE |
Temperature [K] | 100 |
Detector technology | IMAGE PLATE |
Collection date | 2006-02-01 |
Detector | mar345 dtb |
Wavelength(s) | 1.54 |
Spacegroup name | P 63 2 2 |
Unit cell lengths | 60.580, 60.580, 85.065 |
Unit cell angles | 90.00, 90.00, 120.00 |
Refinement procedure
Resolution | 24.940 - 2.100 |
R-factor | 0.25074 |
Rwork | 0.249 |
R-free | 0.29306 |
Structure solution method | SIRAS |
RMSD bond length | 0.011 |
RMSD bond angle | 1.401 |
Data reduction software | DENZO |
Data scaling software | SCALEPACK |
Phasing software | SOLVE |
Refinement software | REFMAC (5.2.0019) |
Data quality characteristics
Overall | Outer shell | |
Low resolution limit [Å] | 25.000 | 2.400 |
High resolution limit [Å] | 2.100 | 2.100 |
Number of reflections | 5816 | |
<I/σ(I)> | 12.9 | 2.3 |
Completeness [%] | 99.6 | 96.8 |
Redundancy | 9.7 | 6.9 |
Crystallization Conditions
crystal ID | method | pH | temperature | details |
1 | VAPOR DIFFUSION, HANGING DROP | 6.5 | 298 | Ammonium sulfate, bis-Tris, PEG3350, pH6.5, vapor diffusion, hanging drop, temperature 298K, VAPOR DIFFUSION, HANGING DROP |