3BSX
Crystal Structure of Human Pumilio 1 in complex with Puf5 RNA
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | NSLS BEAMLINE X4C |
| Synchrotron site | NSLS |
| Beamline | X4C |
| Detector technology | IMAGE PLATE |
| Collection date | 2007-05-05 |
| Detector | MAR scanner 345 mm plate |
| Wavelength(s) | 0.979 |
| Spacegroup name | P 21 21 21 |
| Unit cell lengths | 35.835, 65.642, 313.975 |
| Unit cell angles | 90.00, 90.00, 90.00 |
Refinement procedure
| Resolution | 40.930 - 2.320 |
| R-factor | 0.21845 |
| Rwork | 0.212 |
| R-free | 0.27678 |
| Structure solution method | MOLECULAR REPLACEMENT |
| Starting model (for MR) | 1m8y |
| RMSD bond length | 0.016 |
| RMSD bond angle | 1.568 |
| Data reduction software | DENZO |
| Data scaling software | SCALEPACK |
| Phasing software | PHASER |
| Refinement software | REFMAC (5.2.0005) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 50.000 | 2.400 |
| High resolution limit [Å] | 2.320 | 2.320 |
| Number of reflections | 31513 | |
| <I/σ(I)> | 18.3 | 5.2 |
| Completeness [%] | 94.0 | 84.9 |
| Redundancy | 3.7 | 2.6 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, HANGING DROP | 6.5 | 277 | PEG 8000, 0.05M Sodium Cacodylate pH 6.5, 0.2M KCl, 0.1M Magnesium acetate, VAPOR DIFFUSION, HANGING DROP, temperature 277K |
Crystallization Reagents
| ID | crystal ID | solution ID | reagent name | concentration | details |
| 1 | 1 | 1 | PEG 8000 | ||
| 10 | 1 | 2 | H2O | ||
| 2 | 1 | 1 | Sodium Cacodylate | ||
| 3 | 1 | 1 | KCl | ||
| 4 | 1 | 1 | Magnesium acetate | ||
| 5 | 1 | 1 | H2O | ||
| 6 | 1 | 2 | PEG 8000 | ||
| 7 | 1 | 2 | Sodium Cacodylate | ||
| 8 | 1 | 2 | KCl | ||
| 9 | 1 | 2 | Magnesium acetate |
