3BSB
Crystal Structure of Human Pumilio1 in Complex with CyclinB reverse RNA
Experimental procedure
Experimental method | SINGLE WAVELENGTH |
Source type | SYNCHROTRON |
Source details | APS BEAMLINE 17-ID |
Synchrotron site | APS |
Beamline | 17-ID |
Temperature [K] | 100 |
Detector technology | CCD |
Collection date | 2006-08-04 |
Detector | MAR CCD 165 mm |
Wavelength(s) | 0.979 |
Spacegroup name | P 21 21 21 |
Unit cell lengths | 35.874, 64.292, 321.230 |
Unit cell angles | 90.00, 90.00, 90.00 |
Refinement procedure
Resolution | 25.870 - 2.800 |
R-factor | 0.244 |
Rwork | 0.244 |
R-free | 0.26800 |
Structure solution method | MOLECULAR REPLACEMENT |
Starting model (for MR) | 1m8y |
RMSD bond length | 0.012 |
RMSD bond angle | 1.600 |
Data reduction software | DENZO |
Data scaling software | SCALEPACK |
Phasing software | PHASER |
Refinement software | CNS (1.1) |
Data quality characteristics
Overall | Outer shell | |
Low resolution limit [Å] | 50.000 | 2.900 |
High resolution limit [Å] | 2.800 | 2.800 |
Number of reflections | 30457 | |
<I/σ(I)> | 20.3 | 4.72 |
Completeness [%] | 92.3 | 67.5 |
Redundancy | 6.4 | 6.3 |
Crystallization Conditions
crystal ID | method | pH | temperature | details |
1 | VAPOR DIFFUSION | 6.5 | 277 | Hanging drop, pH 6.5, VAPOR DIFFUSION, temperature 277K |
1 | VAPOR DIFFUSION | 6.5 | 277 | Hanging drop, pH 6.5, VAPOR DIFFUSION, temperature 277K |
Crystallization Reagents
ID | crystal ID | solution ID | reagent name | concentration | details |
1 | 1 | 1 | H2O | ||
2 | 1 | 2 | H2O |