3BSB

Crystal Structure of Human Pumilio1 in Complex with CyclinB reverse RNA

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	SYNCHROTRON
Source details	APS BEAMLINE 17-ID
Synchrotron site	APS
Beamline	17-ID
Temperature [K]	100
Detector technology	CCD
Collection date	2006-08-04
Detector	MAR CCD 165 mm
Wavelength(s)	0.979
Spacegroup name	P 21 21 21
Unit cell lengths	35.874, 64.292, 321.230
Unit cell angles	90.00, 90.00, 90.00

Refinement procedure

Resolution	25.870 - 2.800
R-factor	0.244
Rwork	0.244
R-free	0.26800
Structure solution method	MOLECULAR REPLACEMENT
Starting model (for MR)	1m8y
RMSD bond length	0.012
RMSD bond angle	1.600
Data reduction software	DENZO
Data scaling software	SCALEPACK
Phasing software	PHASER
Refinement software	CNS (1.1)

Data quality characteristics

	Overall	Outer shell
Low resolution limit [Å]	50.000	2.900
High resolution limit [Å]	2.800	2.800
Number of reflections	30457
<I/σ(I)>	20.3	4.72
Completeness [%]	92.3	67.5
Redundancy	6.4	6.3

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	VAPOR DIFFUSION	6.5	277	Hanging drop, pH 6.5, VAPOR DIFFUSION, temperature 277K
1	VAPOR DIFFUSION	6.5	277	Hanging drop, pH 6.5, VAPOR DIFFUSION, temperature 277K

Crystallization Reagents

ID	crystal ID	solution ID	reagent name	concentration	details
1	1	1	H2O
2	1	2	H2O