3BS8
Crystal structure of Glutamate 1-Semialdehyde Aminotransferase complexed with pyridoxamine-5'-phosphate From Bacillus subtilis
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | ROTATING ANODE |
| Source details | RIGAKU MICROMAX-007 HF |
| Temperature [K] | 100 |
| Detector technology | IMAGE PLATE |
| Collection date | 2006-09-06 |
| Detector | MARRESEARCH |
| Wavelength(s) | 1.54179 |
| Spacegroup name | C 1 2 1 |
| Unit cell lengths | 109.251, 55.761, 80.504 |
| Unit cell angles | 90.00, 132.02, 90.00 |
Refinement procedure
| Resolution | 20.000 - 2.300 |
| R-factor | 0.19547 |
| Rwork | 0.193 |
| R-free | 0.23362 |
| Structure solution method | MOLECULAR REPLACEMENT |
| Starting model (for MR) | 3gsb |
| RMSD bond length | 0.007 |
| RMSD bond angle | 1.050 |
| Data reduction software | AUTOMAR |
| Phasing software | MOLREP |
| Refinement software | REFMAC (5.2.0019) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 59.761 | 2.359 |
| High resolution limit [Å] | 2.300 | 2.300 |
| Rmerge | 0.070 | |
| Number of reflections | 15153 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, HANGING DROP | 8.5 | 295 | 100mM Bicine, 30%(w/v) PEG 3350, pH 8.5, VAPOR DIFFUSION, HANGING DROP, temperature 295K |
