3BRQ
Crystal structure of the Escherichia coli transcriptional repressor ascG
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | APS BEAMLINE 19-ID |
| Synchrotron site | APS |
| Beamline | 19-ID |
| Temperature [K] | 100 |
| Detector technology | CCD |
| Collection date | 2006-12-04 |
| Detector | ADSC QUANTUM 315 |
| Wavelength(s) | 0.97935 |
| Spacegroup name | P 21 21 21 |
| Unit cell lengths | 62.229, 65.446, 130.706 |
| Unit cell angles | 90.00, 90.00, 90.00 |
Refinement procedure
| Resolution | 45.080 - 2.000 |
| R-factor | 0.18875 |
| Rwork | 0.186 |
| R-free | 0.24912 |
| Structure solution method | SAD |
| RMSD bond length | 0.018 |
| RMSD bond angle | 1.654 |
| Data reduction software | HKL-2000 |
| Data scaling software | HKL-2000 |
| Phasing software | HKL-3000 |
| Refinement software | REFMAC (5.2.0019) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 50.000 | 2.070 |
| High resolution limit [Å] | 2.000 | 2.000 |
| Rmerge | 0.096 | 0.304 |
| Number of reflections | 63778 | |
| <I/σ(I)> | 37.6 | 6.6 |
| Completeness [%] | 96.2 | 78 |
| Redundancy | 7 | 5.5 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, HANGING DROP | 5.5 | 295 | 0.2M Ammonium sulfate, 0.1M Bis-Tris pH 5.5, 30% PEG MME 2000, 2% Sucrose, VAPOR DIFFUSION, HANGING DROP, temperature 295K |
