3BRP
Crystal Structure of C-Phycocyanin from Galdieria sulphuraria
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | APS BEAMLINE 19-ID |
| Synchrotron site | APS |
| Beamline | 19-ID |
| Temperature [K] | 100 |
| Detector technology | CCD |
| Collection date | 2007-12-08 |
| Detector | ADSC QUANTUM 315 |
| Wavelength(s) | 0.97923 |
| Spacegroup name | H 3 2 |
| Unit cell lengths | 102.818, 102.818, 172.883 |
| Unit cell angles | 90.00, 90.00, 120.00 |
Refinement procedure
| Resolution | 10.000 - 1.850 |
| R-factor | 0.18466 |
| Rwork | 0.182 |
| R-free | 0.23127 |
| Structure solution method | MOLECULAR REPLACEMENT |
| Starting model (for MR) | 1ktp |
| RMSD bond length | 0.016 |
| RMSD bond angle | 2.298 |
| Data reduction software | HKL-3000 |
| Data scaling software | HKL-3000 |
| Phasing software | PHASER |
| Refinement software | REFMAC (5.2.0019) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 50.000 | 1.920 |
| High resolution limit [Å] | 1.850 | 1.850 |
| Rmerge | 0.092 | 0.538 |
| Number of reflections | 30145 | |
| <I/σ(I)> | 15.5 | 2.516 |
| Completeness [%] | 99.5 | 98.4 |
| Redundancy | 5.3 | 5.2 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, HANGING DROP | 6.4 | 291 | 9.5% PEG 2000,0.1M HEPES, 0.1M MGCl2, pH 6.4, VAPOR DIFFUSION, HANGING DROP, temperature 291K |
