3BRO
Crystal structure of the transcription regulator MarR from Oenococcus oeni PSU-1
Experimental procedure
Experimental method | SINGLE WAVELENGTH |
Source type | SYNCHROTRON |
Source details | APS BEAMLINE 19-ID |
Synchrotron site | APS |
Beamline | 19-ID |
Temperature [K] | 100 |
Detector technology | CCD |
Collection date | 2007-10-11 |
Detector | ADSC QUANTUM 315 |
Wavelength(s) | 0.97930 |
Spacegroup name | P 21 21 21 |
Unit cell lengths | 61.489, 80.426, 143.257 |
Unit cell angles | 90.00, 90.00, 90.00 |
Refinement procedure
Resolution | 38.720 - 2.040 |
R-factor | 0.209 |
Rwork | 0.207 |
R-free | 0.24700 |
Structure solution method | SAD |
RMSD bond length | 0.018 |
RMSD bond angle | 1.517 |
Data reduction software | HKL-3000 |
Data scaling software | HKL-3000 |
Phasing software | HKL-3000 |
Refinement software | REFMAC (5.2.0019) |
Data quality characteristics
Overall | Outer shell | |
Low resolution limit [Å] | 50.000 | 2.090 |
High resolution limit [Å] | 2.020 | 2.020 |
Number of reflections | 45048 | |
<I/σ(I)> | 9.5 | 2.4 |
Completeness [%] | 96.8 | 69.9 |
Redundancy | 16.7 | 9.2 |
Crystallization Conditions
crystal ID | method | pH | temperature | details |
1 | VAPOR DIFFUSION, SITTING DROP | 6.5 | 289 | 0.1M Bis-Tris pH 6.5, 28% PEG MME 2000, VAPOR DIFFUSION, SITTING DROP, temperature 289K |