3BRC
Crystal structure of a conserved protein of unknown function from Methanobacterium thermoautotrophicum
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | APS BEAMLINE 19-ID |
| Synchrotron site | APS |
| Beamline | 19-ID |
| Temperature [K] | 100 |
| Detector technology | CCD |
| Collection date | 2007-07-03 |
| Detector | ADSC QUANTUM 315 |
| Wavelength(s) | 0.97940 |
| Spacegroup name | C 1 2 1 |
| Unit cell lengths | 106.894, 48.916, 63.868 |
| Unit cell angles | 90.00, 118.54, 90.00 |
Refinement procedure
| Resolution | 56.080 - 1.600 |
| R-factor | 0.18586 |
| Rwork | 0.183 |
| R-free | 0.23669 |
| Structure solution method | SAD |
| RMSD bond length | 0.013 |
| RMSD bond angle | 1.312 |
| Data reduction software | HKL-3000 |
| Data scaling software | HKL-3000 |
| Phasing software | HKL-3000 |
| Refinement software | REFMAC (5.2.0019) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 88.520 | 1.642 |
| High resolution limit [Å] | 1.600 | 1.600 |
| Rmerge | 0.090 | 0.378 |
| Number of reflections | 32286 | |
| <I/σ(I)> | 27.05 | 1.35 |
| Completeness [%] | 88.5 | 40.92 |
| Redundancy | 4.4 | 2 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, SITTING DROP | 289 | 0.1M Ammonium phosphate, 16% PEG 3350, 2% Glycerol, VAPOR DIFFUSION, SITTING DROP, temperature 289K |
