3BQX
High resolution crystal structure of a glyoxalase-related enzyme from Fulvimarina pelagi
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | APS BEAMLINE 31-ID |
| Synchrotron site | APS |
| Beamline | 31-ID |
| Temperature [K] | 100 |
| Detector technology | CCD |
| Collection date | 2007-12-12 |
| Detector | MAR CCD 165 mm |
| Wavelength(s) | 0.9798 |
| Spacegroup name | C 2 2 21 |
| Unit cell lengths | 53.930, 69.750, 83.070 |
| Unit cell angles | 90.00, 90.00, 90.00 |
Refinement procedure
| Resolution | 17.070 - 1.400 |
| R-factor | 0.238 |
| Rwork | 0.238 |
| R-free | 0.24800 |
| Structure solution method | SAD |
| RMSD bond length | 0.005 |
| RMSD bond angle | 1.400 |
| Data reduction software | MOSFLM |
| Data scaling software | SCALA |
| Phasing software | SHELX |
| Refinement software | CNS (1.1) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 17.070 | 1.390 |
| High resolution limit [Å] | 1.320 | 1.320 |
| Rmerge | 0.060 | 0.861 |
| Number of reflections | 37108 | |
| <I/σ(I)> | 18.3 | 3.8 |
| Completeness [%] | 98.8 | 92.7 |
| Redundancy | 14.3 | 12.9 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, SITTING DROP | 8.5 | 293 | 2.0M Ammonium sulfate, 10 mM Tris-HCl pH 8.5, VAPOR DIFFUSION, SITTING DROP, temperature 293K |
