3BQO

Crystal Structure of TRF1 TRFH domain and TIN2 peptide complex

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	SYNCHROTRON
Source details	APS BEAMLINE 23-ID-D
Synchrotron site	APS
Beamline	23-ID-D
Temperature [K]	100
Detector technology	CCD
Collection date	2006-07-19
Detector	MARMOSAIC 300 mm CCD
Spacegroup name	P 61 2 2
Unit cell lengths	117.105, 117.105, 88.018
Unit cell angles	90.00, 90.00, 120.00

Refinement procedure

Resolution	50.000 - 2.000
Rwork	0.220
R-free	0.23690
Structure solution method	MOLECULAR REPLACEMENT
Starting model (for MR)	1h6o
RMSD bond length	0.006
RMSD bond angle	1.237
Data reduction software	HKL-2000
Data scaling software	HKL-2000
Phasing software	PHASER
Refinement software	CNS (1.1)

Data quality characteristics

	Overall	Outer shell
Low resolution limit [Å]	50.000	2.070
High resolution limit [Å]	2.000	2.000
Rmerge	0.074	0.443
Number of reflections	24517
<I/σ(I)>	50.3	7.4
Completeness [%]	99.9	100
Redundancy	20.2	18.7

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	VAPOR DIFFUSION, HANGING DROP	8.6	289	NaCl 2.5 M MgCl2 0.35 M Tris 0.1 M pH 8.6 DTT 5 mM , VAPOR DIFFUSION, HANGING DROP, temperature 289K