3BQ9
Crystal structure of predicted nucleotide-binding protein from Idiomarina baltica OS145
Experimental procedure
Experimental method | SINGLE WAVELENGTH |
Source type | SYNCHROTRON |
Source details | APS BEAMLINE 31-ID |
Synchrotron site | APS |
Beamline | 31-ID |
Temperature [K] | 77 |
Detector technology | CCD |
Collection date | 2007-12-08 |
Detector | MAR CCD 165 mm |
Wavelength(s) | 0.97961 |
Spacegroup name | P 21 21 2 |
Unit cell lengths | 98.415, 171.857, 57.362 |
Unit cell angles | 90.00, 90.00, 90.00 |
Refinement procedure
Resolution | 20.000 - 1.800 |
R-factor | 0.21152 |
Rwork | 0.210 |
R-free | 0.27128 |
Structure solution method | SAD |
RMSD bond length | 0.009 |
RMSD bond angle | 1.196 |
Data reduction software | HKL-2000 |
Data scaling software | HKL-2000 |
Phasing software | SHELX |
Refinement software | REFMAC (5.3.0034) |
Data quality characteristics
Overall | Outer shell | |
Low resolution limit [Å] | 50.000 | 1.810 |
High resolution limit [Å] | 1.750 | 1.750 |
Rmerge | 0.157 | 0.810 |
Number of reflections | 100102 | |
<I/σ(I)> | 3.7 | 0.7 |
Completeness [%] | 92.3 | 87.8 |
Redundancy | 4.2 | 3.6 |
Crystallization Conditions
crystal ID | method | pH | temperature | details |
1 | VAPOR DIFFUSION, SITTING DROP | 7.5 | 294 | 100mM Hepes pH 7.5, 2M Ammonium sulfate, 10% Glycerol, VAPOR DIFFUSION, SITTING DROP, temperature 294K |