3BQ7
SAM domain of Diacylglycerol Kinase delta1 (E35G)
Experimental procedure
Experimental method | SINGLE WAVELENGTH |
Source type | SYNCHROTRON |
Source details | ALS BEAMLINE 8.2.1 |
Synchrotron site | ALS |
Beamline | 8.2.1 |
Temperature [K] | 100 |
Detector technology | CCD |
Collection date | 2006-09-14 |
Detector | ADSC QUANTUM 210 |
Wavelength(s) | 1.27 |
Spacegroup name | P 32 |
Unit cell lengths | 108.079, 108.079, 33.513 |
Unit cell angles | 90.00, 90.00, 120.00 |
Refinement procedure
Resolution | 54.040 - 2.900 |
R-factor | 0.254 |
Rwork | 0.250 |
R-free | 0.29000 |
Starting model (for MR) | 2f3n |
Data reduction software | DENZO |
Data scaling software | SCALEPACK |
Phasing software | PHASER |
Refinement software | CNS |
Data quality characteristics
Overall | Inner shell | Outer shell | |
Low resolution limit [Å] | 90.000 | 90.000 | 3.000 |
High resolution limit [Å] | 2.900 | 6.250 | 2.900 |
Rmerge | 0.064 | 0.051 | 0.344 |
Number of reflections | 18986 | ||
<I/σ(I)> | 17.6 | ||
Completeness [%] | 98.1 | 98.8 | 95.4 |
Redundancy | 2 | 2.1 | 1.9 |
Crystallization Conditions
crystal ID | method | pH | temperature | details |
1 | VAPOR DIFFUSION, HANGING DROP | 8 | 298 | dibasic ammonium phosphate, tris, NaCl, beta-mercaptoethanol, pH 8.0, vapor diffusion, hanging drop, temperature 298K |