3BQ4
Crystal Structure of Ad35 fiber knob
Experimental procedure
Experimental method | SINGLE WAVELENGTH |
Source type | SYNCHROTRON |
Source details | APS BEAMLINE 23-ID-D |
Synchrotron site | APS |
Beamline | 23-ID-D |
Wavelength(s) | 0.979 |
Spacegroup name | I 4 |
Unit cell lengths | 173.906, 173.906, 154.145 |
Unit cell angles | 90.00, 90.00, 90.00 |
Refinement procedure
Resolution | 46.030 - 2.700 |
R-factor | 0.332 |
Rwork | 0.332 |
R-free | 0.35200 |
Structure solution method | MOLECULAR REPLACEMENT |
RMSD bond length | 0.010 |
RMSD bond angle | 1.700 |
Data reduction software | HKL-2000 |
Data scaling software | HKL-2000 |
Phasing software | PHASER |
Refinement software | CNS (1.2) |
Data quality characteristics
Overall | |
Low resolution limit [Å] | 50.000 |
High resolution limit [Å] | 2.700 |
Rmerge | 0.149 |
Number of reflections | 85255 |
<I/σ(I)> | 13.3 |
Crystallization Conditions
crystal ID | method | pH | temperature | details |
1 | VAPOR DIFFUSION, HANGING DROP | 6.5 | 295.5 | 1.6 M Ammonium sulfate and 0.1 M MES, pH 6.5, VAPOR DIFFUSION, HANGING DROP, temperature 295.5K |