3BQ3
Crystal structure of S. cerevisiae Dcn1
Experimental procedure
| Experimental method | MAD |
| Source type | SYNCHROTRON |
| Source details | APS BEAMLINE 8-BM |
| Synchrotron site | APS |
| Beamline | 8-BM |
| Detector technology | CCD |
| Collection date | 2005-11-28 |
| Detector | ADSC QUANTUM 315 |
| Spacegroup name | C 1 2 1 |
| Unit cell lengths | 106.863, 51.453, 70.852 |
| Unit cell angles | 90.00, 129.82, 90.00 |
Refinement procedure
| Resolution | 29.480 - 1.900 |
| R-factor | 0.2 |
| Rwork | 0.197 |
| R-free | 0.24300 |
| RMSD bond length | 0.020 |
| RMSD bond angle | 1.715 |
| Data reduction software | DENZO |
| Data scaling software | SCALEPACK |
| Refinement software | REFMAC (5.2.0005) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 50.000 | 1.970 |
| High resolution limit [Å] | 1.900 | 1.900 |
| Rmerge | 0.052 | 0.479 |
| Number of reflections | 21992 | |
| <I/σ(I)> | 22.28 | 2.412 |
| Completeness [%] | 97.6 | 83.7 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, HANGING DROP | 5.6 | 293 | 400mM Ammonium Acetate, 30% PEG 4000 (w/v), 0.1M Tri-Sodium Citrate, pH 5.6, VAPOR DIFFUSION, HANGING DROP, temperature 293K |
