3BQ2
Post-insertion binary complex of Dbh DNA polymerase
Experimental procedure
Experimental method | SINGLE WAVELENGTH |
Source type | SYNCHROTRON |
Source details | NSLS BEAMLINE X25 |
Synchrotron site | NSLS |
Beamline | X25 |
Temperature [K] | 100 |
Detector technology | CCD |
Collection date | 2006-07-14 |
Detector | ADSC QUANTUM 315 |
Wavelength(s) | 0.9795 |
Spacegroup name | P 41 21 2 |
Unit cell lengths | 120.338, 120.338, 68.325 |
Unit cell angles | 90.00, 90.00, 90.00 |
Refinement procedure
Resolution | 20.000 - 2.700 |
R-factor | 0.23704 |
Rwork | 0.234 |
R-free | 0.28906 |
Structure solution method | MOLECULAR REPLACEMENT |
Starting model (for MR) | PDB entries 1IM4 and 1K1Q |
RMSD bond length | 0.011 |
RMSD bond angle | 1.328 |
Data reduction software | DENZO |
Data scaling software | SCALEPACK |
Phasing software | PHASER |
Refinement software | REFMAC (5.2.0019) |
Data quality characteristics
Overall | Outer shell | |
Low resolution limit [Å] | 25.000 | 2.750 |
High resolution limit [Å] | 2.700 | 2.700 |
Rmerge | 0.070 | 0.444 |
Number of reflections | 13596 | |
<I/σ(I)> | 16.6 | 2 |
Completeness [%] | 95.0 | 91.9 |
Redundancy | 5.3 | 3.7 |
Crystallization Conditions
crystal ID | method | pH | temperature | details |
1 | VAPOR DIFFUSION, HANGING DROP | 7 | 293 | PEG-3350, calcium acetate, glycerol, HEPES, pH 7, VAPOR DIFFUSION, HANGING DROP, temperature 293K |
Crystallization Reagents
ID | crystal ID | solution ID | reagent name | concentration | details |
1 | 1 | 1 | PEG-3350 | ||
2 | 1 | 2 | PEG-3350 | ||
3 | 1 | 1 | calcium acetate | ||
4 | 1 | 1 | calcium acetate | ||
5 | 1 | 1 | glycerol | ||
6 | 1 | 2 | glycerol | ||
7 | 1 | 1 | HEPES | ||
8 | 1 | 2 | HEPES |