3BPQ
Crystal Structure of RelB-RelE antitoxin-toxin complex from Methanococcus jannaschii
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | BESSY BEAMLINE 14.1 |
| Synchrotron site | BESSY |
| Beamline | 14.1 |
| Temperature [K] | 100 |
| Detector technology | CCD |
| Collection date | 2006-07-12 |
| Detector | MAR CCD 165 mm |
| Wavelength(s) | 0.94905 |
| Spacegroup name | P 1 21 1 |
| Unit cell lengths | 52.794, 57.992, 58.747 |
| Unit cell angles | 90.00, 92.30, 90.00 |
Refinement procedure
| Resolution | 20.000 - 2.200 |
| R-factor | 0.24007 |
| Rwork | 0.238 |
| R-free | 0.27418 |
| Structure solution method | MIR |
| RMSD bond length | 0.008 |
| RMSD bond angle | 1.058 |
| Data reduction software | HKL-2000 |
| Data scaling software | SCALEPACK |
| Phasing software | SHARP |
| Refinement software | REFMAC (5.2.0019) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 50.000 | 2.180 |
| High resolution limit [Å] | 2.130 | 2.130 |
| Rmerge | 0.050 | 0.421 |
| Number of reflections | 19952 | |
| <I/σ(I)> | 15.4 | 3.13 |
| Completeness [%] | 99.6 | 98.9 |
| Redundancy | 4.2 | 4.1 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, HANGING DROP | 5.5 | 298 | 20%MPD, 0.1M Bis-Tris pH5.5, VAPOR DIFFUSION, HANGING DROP, temperature 298K |
