3BPQ
Crystal Structure of RelB-RelE antitoxin-toxin complex from Methanococcus jannaschii
Experimental procedure
Experimental method | SINGLE WAVELENGTH |
Source type | SYNCHROTRON |
Source details | BESSY BEAMLINE 14.1 |
Synchrotron site | BESSY |
Beamline | 14.1 |
Temperature [K] | 100 |
Detector technology | CCD |
Collection date | 2006-07-12 |
Detector | MAR CCD 165 mm |
Wavelength(s) | 0.94905 |
Spacegroup name | P 1 21 1 |
Unit cell lengths | 52.794, 57.992, 58.747 |
Unit cell angles | 90.00, 92.30, 90.00 |
Refinement procedure
Resolution | 20.000 - 2.200 |
R-factor | 0.24007 |
Rwork | 0.238 |
R-free | 0.27418 |
Structure solution method | MIR |
RMSD bond length | 0.008 |
RMSD bond angle | 1.058 |
Data reduction software | HKL-2000 |
Data scaling software | SCALEPACK |
Phasing software | SHARP |
Refinement software | REFMAC (5.2.0019) |
Data quality characteristics
Overall | Outer shell | |
Low resolution limit [Å] | 50.000 | 2.180 |
High resolution limit [Å] | 2.130 | 2.130 |
Rmerge | 0.050 | 0.421 |
Number of reflections | 19952 | |
<I/σ(I)> | 15.4 | 3.13 |
Completeness [%] | 99.6 | 98.9 |
Redundancy | 4.2 | 4.1 |
Crystallization Conditions
crystal ID | method | pH | temperature | details |
1 | VAPOR DIFFUSION, HANGING DROP | 5.5 | 298 | 20%MPD, 0.1M Bis-Tris pH5.5, VAPOR DIFFUSION, HANGING DROP, temperature 298K |