Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | SPRING-8 BEAMLINE BL26B1 |
| Synchrotron site | SPring-8 |
| Beamline | BL26B1 |
| Temperature [K] | 100 |
| Detector technology | CCD |
| Collection date | 2006-04-18 |
| Detector | ADSC QUANTUM 4 |
| Wavelength(s) | 1.000 |
| Spacegroup name | C 1 2 1 |
| Unit cell lengths | 99.635, 76.469, 62.790 |
| Unit cell angles | 90.00, 109.06, 90.00 |
Refinement procedure
| Resolution | 38.240 - 1.700 |
| R-factor | 0.20004 |
| Rwork | 0.198 |
| R-free | 0.24629 |
| Structure solution method | MOLECULAR REPLACEMENT |
| Starting model (for MR) | 1wmq |
| RMSD bond length | 0.014 |
| RMSD bond angle | 1.560 |
| Data reduction software | HKL-2000 |
| Data scaling software | HKL-2000 |
| Phasing software | MOLREP |
| Refinement software | REFMAC (5.2.0019) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 40.000 | 1.760 |
| High resolution limit [Å] | 1.700 | 1.700 |
| Rmerge | 0.045 | 0.362 |
| Number of reflections | 48341 | |
| Completeness [%] | 99.4 | 98.4 |
| Redundancy | 3.6 | 3.3 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, HANGING DROP | 7.4 | 293 | 40% MPD, 0.1M HEPES, 0.1M MGCL2, pH 7.40, VAPOR DIFFUSION, HANGING DROP, temperature 293K |
Crystallization Reagents
| ID | crystal ID | solution ID | reagent name | concentration | details |
| 1 | 1 | 1 | MPD | ||
| 2 | 1 | 1 | HEPES | ||
| 3 | 1 | 1 | MgCl2 | ||
| 4 | 1 | 1 | H2O | ||
| 5 | 1 | 2 | MPD | ||
| 6 | 1 | 2 | HEPES | ||
| 7 | 1 | 2 | MgCl2 | ||
| 8 | 1 | 2 | H2O |
