3BNV
Crystal structure of Cj0977, a sigma28-regulated virulence protein from Campylobacter jejuni.
Experimental procedure
| Experimental method | MAD |
| Source type | SYNCHROTRON |
| Source details | APS BEAMLINE 19-BM |
| Synchrotron site | APS |
| Beamline | 19-BM |
| Temperature [K] | 100 |
| Detector technology | CCD |
| Detector | SBC3 3k X 3k CCD |
| Wavelength(s) | 0.97929,0.979450,0.971620 |
| Spacegroup name | P 1 21 1 |
| Unit cell lengths | 79.876, 94.533, 81.818 |
| Unit cell angles | 90.00, 99.32, 90.00 |
Refinement procedure
| Resolution | 37.930 - 2.600 |
| R-factor | 0.21589 |
| Rwork | 0.213 |
| R-free | 0.26925 |
| Structure solution method | MAD |
| RMSD bond length | 0.014 |
| RMSD bond angle | 1.433 |
| Data reduction software | HKL-3000 |
| Data scaling software | SCALEPACK |
| Phasing software | SHARP |
| Refinement software | REFMAC (5.2.0019) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 37.930 | 2.690 |
| High resolution limit [Å] | 2.600 | 2.600 |
| Rmerge | 0.098 | 0.339 |
| Number of reflections | 36505 | |
| <I/σ(I)> | 7.1 | |
| Completeness [%] | 95.6 | 68.3 |
| Redundancy | 6.43 | 2.5 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, HANGING DROP | 7.2 | 285 | 26% PEG4000, 0.2M ammonium acetate, 0.1M Tris-HCl, pH 7.2, VAPOR DIFFUSION, HANGING DROP, temperature 285K |
